Remember at all times - If the discrepancy is not resolved and transfusion is required STAT, select group O RBC components and AB plasma products.

  1. Recheck suitability of specimen(s). Determine specimen suitability by checking for contamination with IV fluid, unusual color or appearance, etc.

    If there is any doubt about the identity or the quality of the specimen, collect a new specimen and repeat the ABO grouping.

  2. Check the label on the reagent vial(s) to ensure that the correct reagents were used in initial testing.
  3. Recheck reagent appearance for possible contamination by comparing it with an unopened vial of the same reagent (and lot number, if possible). Use a new reagent vial if the current vial appears contaminated.
  4. Prepare a new 3% patient cell suspension.
  5. Repeat the ABO grouping.
  6. Obtain and review the patient diagnosis and an extended transfusion/ obstetrical history. Document the number and ABO of the units transfused, if applicable. If required call other facilities the patient was recently admitted to get a thorough transfusion history.