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 Discussion 

Question 1. What additional testing should be done on the specimen?
 

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Question 2. What is the minimal positive grading for the reverse grouping cells to be considered positive?
 

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Question 3. What is the probable reason for this discrepancy?
 

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Question 4. What is the follow-up when, after performing routine investigation, a valid reverse group cannot be obtained or explained?
 

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Question 5.  When a reverse grouping remains un resolved (i.e., reactions in the reverse group are still less than 2+) what additional testing must always be performed when crossmatched blood is required?
 

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